Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Beta-HPV 5 and 8 E6 promote p300 degradation by blocking AKT/p300 association.
The E6 oncoprotein from high-risk genus alpha human papillomaviruses (α-HPVs), such as HPV 16, has been well characterized with respect to the host-cell proteins it interacts with and corresponding signaling pathways that are disrupted due to these interactions. Less is known regarding the interacting partners of E6 from the genus beta papillomaviruses (β-HPVs); however, it is generally thought that β-HPV ... [more]
PLoS Pathog. Aug. 01, 2011; 7(8);e1002211 [Pubmed: 21901101]
Throughput
- High Throughput
Ontology Terms
- prepuce (BTO:0001113)
- foreskin keratinocyte (CL:1001606)
- keratinocyte cell line (BTO:0000375)
Additional Notes
- HFK
Curated By
- BioGRID