BAIT

E6

E6 protein, VE6_HPV18
Protein E6
Human Papilloma Virus (HPV) Project
GO Process (0)
GO Function (0)
GO Component (0)
Human papillomavirus (18)
PREY

VPS35

MEM3, PARK17, TCCCTA00141
vacuolar protein sorting 35 homolog (S. cerevisiae)
Alzheimer's Disease Project
Parkinson's Disease Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins.

Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Muenger K, Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M

Genotypic differences greatly influence susceptibility and resistance to disease. Understanding genotype-phenotype relationships requires that phenotypes be viewed as manifestations of network properties, rather than simply as the result of individual genomic variations. Genome sequencing efforts have identified numerous germline mutations, and large numbers of somatic genomic alterations, associated with a predisposition to cancer. However, it remains difficult to distinguish background, ... [more]

Nature Jul. 26, 2012; 487(7408);491-5 [Pubmed: 22810586]

Throughput

  • High Throughput

Ontology Terms

  • imr-90 cell (BTO:0001229) [lung fibroblast cell line (BTO:0000161)]
  • fibroblast (CL:0000057)
  • lung (BTO:0000763)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
E6 VPS35
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
3781986

Curated By

  • BioGRID