An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

TopBP1 assembles nuclear condensates to switch on ATR signaling.

Frattini C, Promonet A, Alghoul E, Vidal-Eychenie S, Lamarque M, Blanchard MP, Urbach S, Basbous J, Constantinou A

ATR checkpoint signaling is crucial for cellular responses to DNA replication impediments. Using an optogenetic platform, we show that TopBP1, the main activator of ATR, self-assembles extensively to yield micrometer-sized condensates. These opto-TopBP1 condensates are functional entities organized in tightly packed clusters of spherical nano-particles. TopBP1 condensates are reversible, occasionally fuse, and co-localize with TopBP1 partner proteins. We provide evidence ... [more]

Mol Cell Mar. 18, 2021; 81(6);1231-1245.e8 [Pubmed: 33503405]

Throughput

High Throughput

Additional Notes

BioID

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TOPBP1 HDLBP	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Havugimana PC (2012)	High	0.787	BioGRID	752014

Curated By

BioGRID

Interaction Summary

TOPBP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]protein C-terminus binding [TAS]protein binding [IPI]

Gene Ontology Cellular Component

HDLBP

Gene Ontology Molecular Function

lipid binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]