BAIT

TOPBP1

TOP2BP1
topoisomerase (DNA) II binding protein 1
Fanconi Anemia Project
Human Papilloma Virus (HPV) Project
Homo sapiens
PREY

HNRNPA3

2610510D13Rik, D10S102, FBRNP, HNRPA3
heterogeneous nuclear ribonucleoprotein A3
GO Process (3)
GO Function (3)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

TopBP1 assembles nuclear condensates to switch on ATR signaling.

Frattini C, Promonet A, Alghoul E, Vidal-Eychenie S, Lamarque M, Blanchard MP, Urbach S, Basbous J, Constantinou A

ATR checkpoint signaling is crucial for cellular responses to DNA replication impediments. Using an optogenetic platform, we show that TopBP1, the main activator of ATR, self-assembles extensively to yield micrometer-sized condensates. These opto-TopBP1 condensates are functional entities organized in tightly packed clusters of spherical nano-particles. TopBP1 condensates are reversible, occasionally fuse, and co-localize with TopBP1 partner proteins. We provide evidence ... [more]

Mol Cell Mar. 18, 2021; 81(6);1231-1245.e8 [Pubmed: 33503405]

Throughput

  • High Throughput

Additional Notes

  • BioID

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TOPBP1 HNRNPA3
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3856383

Curated By

  • BioGRID