An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Molecular basis for C-degron recognition by CRL2APPBP2 ubiquitin ligase.

Zhao S, Olmayev-Yaakobov D, Ru W, Li S, Chen X, Zhang J, Yao X, Koren I, Zhang K, Xu C

E3 ubiquitin ligases determine the specificity of eukaryotic protein degradation by selective binding to destabilizing protein motifs, termed degrons, in substrates for ubiquitin-mediated proteolysis. The exposed C-terminal residues of proteins can act as C-degrons that are recognized by distinct substrate receptors (SRs) as part of dedicated cullin-RING E3 ubiquitin ligase (CRL) complexes. APPBP2, an SR of Cullin 2-RING ligase (CRL2), ... [more]

Proc Natl Acad Sci U S A Oct. 24, 2023; 120(43);e2308870120 [Pubmed: 37844242]

Throughput

Low Throughput

Additional Notes

E2: UBE2R1

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
APPBP2 MRPL28	Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.	Zhao S (2023)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

APPBP2

Gene Ontology Biological Process

Gene Ontology Molecular Function

microtubule motor activity [TAS]protein binding [IPI]

Gene Ontology Cellular Component

MRPL28

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]structural constituent of ribosome [NAS]