Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

MARCH7-mediated ubiquitination decreases the solubility of ATG14 to inhibit autophagy.

Shi X, Wu W, Feng Z, Fan P, Shi R, Zhang X

Autophagy is a fundamental biological process critical to all eukaryotic cellular life. Although autophagy has been increasingly studied, how its process is precisely coordinated remains an open question. ATG14 (ATG14L/Barkor) is known to play a crucial role in both autophagosome formation and autophagosome-lysosome fusion. However, how ATG14 is regulated, especially at the post-translation level, is still not clear. Here, we ... [more]

Cell Rep Sep. 26, 2023; 42(9);113045 [Pubmed: 37632749]

Throughput

  • High Throughput

Curated By

  • BioGRID