BAIT

RHOBTB1

Rho-related BTB domain containing 1
UPS Project
GO Process (2)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

HNRNPA3

2610510D13Rik, D10S102, FBRNP, HNRPA3
heterogeneous nuclear ribonucleoprotein A3
GO Process (3)
GO Function (3)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Structure and Function of RhoBTB1 Required for Substrate Specificity and Cullin-3 Ubiquitination.

Kumar G, Fang S, Golosova D, Lu KT, Brozoski DT, Vazirabad I, Sigmund CD

We identified Rho-related BTB domain containing 1 (RhoBTB1) as a key regulator of phosphodiesterase 5 (PDE5) activity, and through PDE5, a regulator of vascular tone. We identified the binding interface for PDE5 on RhoBTB1 by truncating full-length RhoBTB1 into its component domains. Co-immunoprecipitation analyses revealed that the C-terminal half of RhoBTB1 containing its two BTB domains and the C-terminal domain ... [more]

Function (Oxf) Aug. 14, 2023; 4(5);zqad034 [Pubmed: 37575477]

Throughput

  • High Throughput

Additional Notes

  • Proximity label MS was carried out to identify high confidence protein interactors of RhoBTB1 B1B2C or B1B2 (p-value <0.05)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RHOBTB1 HNRNPA3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3336001

Curated By

  • BioGRID