Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Experimental and computational evidence that Calpain-10 binds to the carboxy terminus of NaV1.2 and NaV1.6.

Arratia LM, Bermudes-Contreras JD, Juarez-Monroy JA, Romero-Macias EA, Luna-Rojas JC, Lopez-Hidalgo M, Vega AV, Zamorano-Carrillo A

Voltage-gated sodium channels (NaV) are pivotal proteins responsible for initiating and transmitting action potentials. Emerging evidence suggests that proteolytic cleavage of sodium channels by calpains is pivotal in diverse physiological scenarios, including ischemia, brain injury, and neuropathic pain associated with diabetes. Despite this significance, the precise mechanism by which calpains recognize sodium channels, especially given the multiple calpain isoforms expressed ... [more]

Sci Rep Mar. 21, 2024; 14(1);6761 [Pubmed: 38514708]

Throughput

  • High Throughput

Curated By

  • BioGRID