BAIT

POB3

FACT complex subunit POB3, L000003326, YML069W
Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

SSB1

YG101, Hsp70 family ATPase SSB1, L000002073, L000002508, YDL229W
Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

TAP-MS analysis of FACT interactions and regulation by a ubiquitin ligase, San1.

Barman P, Chakraborty P, Guha S, Kaja A, Bhaumik R, Bhaumik SR

An evolutionarily conserved heterodimeric FACT (Facilitates chromatin transcription) regulates transcription, DNA repair, replication and other cellular processes via its interactions with other proteins. FACT is recently found to be regulated via ubiquitylation and 26S proteasomal degradation, alteration of which is associated with aberrant transcription and genome integrity. However, there has not been a systematic study to analyze FACT interactions proteome-wide ... [more]

Biochim Biophys Acta Gene Regul Mech Mar. 01, 2025; 1868(1);195077 [Pubmed: 39855624]

Throughput

  • High Throughput

Additional Notes

  • High confidence Pob3 interactors in the insoluble fractions (FDR < 1%)..
  • High confidence Pob3 interactors in the soluble fractions (FDR < 1%)..

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SSB1 POB3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
333685
POB3 SSB1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
333686
SSB1 POB3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1375BioGRID
2033390

Curated By

  • BioGRID