BAIT

POB3

FACT complex subunit POB3, L000003326, YML069W
Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

MED11

L000004634, YMR112C
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

TAP-MS analysis of FACT interactions and regulation by a ubiquitin ligase, San1.

Barman P, Chakraborty P, Guha S, Kaja A, Bhaumik R, Bhaumik SR

An evolutionarily conserved heterodimeric FACT (Facilitates chromatin transcription) regulates transcription, DNA repair, replication and other cellular processes via its interactions with other proteins. FACT is recently found to be regulated via ubiquitylation and 26S proteasomal degradation, alteration of which is associated with aberrant transcription and genome integrity. However, there has not been a systematic study to analyze FACT interactions proteome-wide ... [more]

Biochim Biophys Acta Gene Regul Mech Mar. 01, 2025; 1868(1);195077 [Pubmed: 39855624]

Throughput

  • High Throughput

Additional Notes

  • High confidence Pob3 interactors in the insoluble fractions (FDR < 1%)..

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MED11 POB3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.134BioGRID
1947054

Curated By

  • BioGRID