PREY

ATP6V1E2

ATP6E1, ATP6EL2, ATP6V1EL2, VMA4
ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2
GO Process (6)
GO Function (0)
GO Component (1)
Homo sapiens

Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

EndoMAP.v1 charts the structural landscape of human early endosome complexes.

Gonzalez-Lozano MA, Schmid EW, Miguel Whelan E, Jiang Y, Paulo JA, Walter JC, Harper JW

Early or sorting endosomes are dynamic organelles that play key roles in proteome control by triaging plasma membrane proteins for either recycling or degradation in the lysosome1,2. These events are coordinated by numerous transiently associated regulatory complexes and integral membrane components that contribute to organelle identity during endosome maturation3. Although a subset of the several hundred protein components and cargoes ... [more]

Nature May. 28, 2025; (); [Pubmed: 40437099]

Throughput

  • High Throughput

Additional Notes

  • Blue-native polyacrylamide gel co-fractionation-MS (BN-MS)
  • High confidence protein interactions had a PCProphet score > 0.7 in at least two replicates.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ATP6V1D ATP6V1E2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
3075339

Curated By

  • BioGRID