ATP5B
Gene Ontology Biological Process
- ATP biosynthetic process [IMP]
- ATP catabolic process [IDA]
- angiogenesis [IMP]
- cellular metabolic process [TAS]
- generation of precursor metabolites and energy [NAS]
- mitochondrial ATP synthesis coupled proton transport [IC, TAS]
- osteoblast differentiation [IDA]
- proton transport [IMP]
- regulation of intracellular pH [IMP]
- respiratory electron transport chain [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell surface [IDA]
- extracellular vesicular exosome [IDA]
- membrane [IDA]
- mitochondrial matrix [NAS, TAS]
- mitochondrial membrane [IDA]
- mitochondrial nucleoid [IDA]
- mitochondrial proton-transporting ATP synthase complex [IDA]
- mitochondrial proton-transporting ATP synthase, catalytic core [NAS]
- mitochondrion [IDA]
- nucleus [IDA]
- plasma membrane [IDA]
ATP5O
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Cross-Linking-MS (XL-MS)
An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).
Publication
EndoMAP.v1 charts the structural landscape of human early endosome complexes.
Early or sorting endosomes are dynamic organelles that play key roles in proteome control by triaging plasma membrane proteins for either recycling or degradation in the lysosome1,2. These events are coordinated by numerous transiently associated regulatory complexes and integral membrane components that contribute to organelle identity during endosome maturation3. Although a subset of the several hundred protein components and cargoes ... [more]
Throughput
- High Throughput
Additional Notes
- High confidence protein interactions had an XlinkX score >40. Re-analysis performed with Scout used a 1% FDR cutoff on Residue Pair level.
- XL-MS of two independent replicates cross-linked with DSSO in HEK293 cells.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ATP5B ATP5O | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9543 | BioGRID | 2251027 | |
| ATP5B ATP5O | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9974 | BioGRID | 3247381 | |
| ATP5B ATP5O | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9196 | BioGRID | 3034857 | |
| ATP5B ATP5O | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.857 | BioGRID | 743991 | |
| ATP5B ATP5O | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 1.6063 | BioGRID | 2625705 | |
| ATP5B ATP5O | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 1 | BioGRID | 1259181 | |
| ATP5B ATP5O | Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071). | High | - | BioGRID | - | |
| ATP5O ATP5B | Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071). | High | - | BioGRID | 3744883 | |
| ATP5B ATP5O | Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071). | High | - | BioGRID | 3676723 |
Curated By
- BioGRID