BAIT

NSP12

ORF1ab, ORF1ab-nsp12, SARS-CoV2 nsp12, SARS-CoV-2 nsp12, RNA-directed RNA polymerase, R1AB_SARS2, PRO_0000449629, GU280_gp01_nsp12
RNA-directed RNA polymerase; Non-structural protein 12
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

RAVER1

hCG_2033729
ribonucleoprotein, PTB-binding 1
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids.

Broderick K, Moutaoufik MT, Saccon T, Malty R, Amin S, Phanse S, Joseph TP, Zilocchi M, Hosseinnia A, Istace Z, Hajikarimlou M, Abrar S, Fisher J, Brassard R, Perera R, Kumar A, Aoki H, Rahmatbakhsh M, Jessulat M, Kobasa D, Dehne F, Prasad B, Gagarinova A, Joanne Lemieux M, Cochrane A, Houry WA, Aly KA, Golshani A, Babu M

Understanding SARS-CoV-2 human protein-protein interactions (PPIs) and the host response to infection is essential for developing effective COVID-19 antivirals. However, how the ancestral virus and its variants remodel virus-host protein assemblies in various organ-specific cells and bodily fluids remains unclear. Here, we conduct 639 affinity-purifications by tagging and expressing 28 SARS-CoV-2 and spike proteins from the ancestral virus and four ... [more]

Nat Commun Jul. 01, 2025; 16(1);5784 [Pubmed: 40593736]

Throughput

  • High Throughput

Ontology Terms

  • hek-293 cell (BTO:0000007) [kidney (BTO:0000671)]

Additional Notes

  • RF score threshold > 0.8

Curated By

  • BioGRID