BAIT

NSP14

ORF1ab, ORF1ab-nsp14, SARS-CoV2 nsp14, SARS-CoV-2 nsp14, Guanine-N7 methyltransferase, exonuclease, R1AB_SARS2, PRO_0000449631, GU280_gp01_nsp14
Guanine-N7 methyltransferase; Non-structural protein 14; 3-to-5 exonuclease
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

EIF2S1

EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A
eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Human Papilloma Virus (HPV) Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids.

Broderick K, Moutaoufik MT, Saccon T, Malty R, Amin S, Phanse S, Joseph TP, Zilocchi M, Hosseinnia A, Istace Z, Hajikarimlou M, Abrar S, Fisher J, Brassard R, Perera R, Kumar A, Aoki H, Rahmatbakhsh M, Jessulat M, Kobasa D, Dehne F, Prasad B, Gagarinova A, Joanne Lemieux M, Cochrane A, Houry WA, Aly KA, Golshani A, Babu M

Understanding SARS-CoV-2 human protein-protein interactions (PPIs) and the host response to infection is essential for developing effective COVID-19 antivirals. However, how the ancestral virus and its variants remodel virus-host protein assemblies in various organ-specific cells and bodily fluids remains unclear. Here, we conduct 639 affinity-purifications by tagging and expressing 28 SARS-CoV-2 and spike proteins from the ancestral virus and four ... [more]

Nat Commun Jul. 01, 2025; 16(1);5784 [Pubmed: 40593736]

Throughput

  • High Throughput

Ontology Terms

  • hek-293 cell (BTO:0000007) [kidney (BTO:0000671)]

Additional Notes

  • RF score threshold > 0.8

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NSP14 EIF2S1
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High0.99BioGRID
3214805
NSP14 EIF2S1
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High16.49BioGRID
2791833

Curated By

  • BioGRID