BAIT

VPS4

CSC1, DID6, END13, GRD13, VPL4, VPT10, AAA family ATPase VPS4, L000002956, YPR173C
AAA-ATPase involved in multivesicular body (MVB) protein sorting; ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
Saccharomyces cerevisiae (S288c)
PREY

LRO1

phospholipid:diacylglycerol acyltransferase, S000007453, YNR008W
Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; Lro1p and Dga1p can O-acylate ceramides; putative homolog of human lecithin cholesterol acyltransferase
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Any1 is a phospholipid scramblase involved in endosome biogenesis.

Gao J, Franzkoch R, Rocha-Roa C, Psathaki OE, Hensel M, Vanni S, Ungermann C

Endosomes are central organelles in the recycling and degradation of receptors and membrane proteins. Once endocytosed, such proteins are sorted at endosomes into intraluminal vesicles (ILVs). The resulting multivesicular bodies (MVBs) then fuse with the lysosomes, leading to the degradation of ILVs and recycling of the resulting monomers. However, the biogenesis of MVBs requires a constant lipid supply for efficient ... [more]

J Cell Biol Apr. 07, 2025; 224(4); [Pubmed: 40047640]

Throughput

  • Low Throughput

Ontology Terms

  • protein transport (APO:0000129)

Additional Notes

  • Genetic complex
  • vsp4 mutation alters Cps1 trafficking (endolysosomal protein trafficking) in Dga1/ Lro1/ Are1 / Are2 quadruple mutants

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LRO1 VPS4
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1333BioGRID
2176626

Curated By

  • BioGRID