BAIT
PIP82
CG11219, Dmel\CG11219, Dmel_CG11219
CG11219 gene product from transcript CG11219-RA
GO Process (1)
GO Function (0)
GO Component (0)
Gene Ontology Biological Process
Drosophila melanogaster
PREY
CG45186
CG1141, CG12790, CG13797, CG13798, CG13799, CG13800, CG16763, CG16764, CG32306, CG33231, CG33232, CG42669, Dmel\CG45186, Dmel_CG13800, Dmel_CG32306, Dmel_CG33231, Dmel_CG33232, Dmel_CG42669, c62E-11, Dmel_CG45186
CG45186 gene product from transcript CG45186-RT
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Drosophila melanogaster
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Proximity labeling reveals interactions necessary to maintain the distinct apical domains of Drosophila photoreceptors.
Specialized membrane and cortical protein regions are common features of cells and are utilized to isolate differential cellular functions. In Drosophila photoreceptors, the apical membrane domain is defined by two distinct morphological membranes: the rhabdomere microvilli and the stalk membrane. To define the apical cortical protein complexes, we performed proximity labeling screens utilizing the rhabdomeric-specific protein PIP82 as bait. We ... [more]
J Cell Sci Dec. 01, 2024; 137(23); [Pubmed: 39540276]
Throughput
- High Throughput
Additional Notes
- Hit in Adult fly retina: 354 fold over control
- Hit in S2 cells: SAINT P = 0.89
- Proximity label MS was carried out to identify high confidence protein interactors in S2 cells with a SAINT P of greater than or equal to 0.8
- Proximity label MS was carried out to identify high confidence protein interactors in adult fly retinas that are enriched greater than or equal 3 fold over control
Curated By
- BioGRID