Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
HERD-1 mediates multiphase condensate immiscibility to regulate small RNA-driven transgenerational epigenetic inheritance.
Biomolecular condensates, such as the nucleolus, stress granules/processing bodies and germ granules, are multiphase assemblages whose formation mechanisms and significance remain poorly understood. Here we identify protein constituents of the spatiotemporally ordered P, Z and M multiphase condensates in Caenorhabditis elegans germ granules using optimized TurboID-mediated proximity biotin labelling. These include 462, 41 and 86 proteins localizing to P, Z ... [more]
Nat Cell Biol Nov. 01, 2024; 26(11);1958-1970 [Pubmed: 39354132]
Throughput
- High Throughput
Additional Notes
- Proximity Label MS was carried out to identify high confidence protein interactors with enrichment ratios greater than or equal to 1.5 and P < 0.05 on all four replicates
- p-value = 1.07E-07
Curated By
- BioGRID