Publication

Affinity Purification Mass Spectrometry on the Orbitrap-Astral Mass Spectrometer Enables High-Throughput Protein-Protein Interaction Mapping.

Serrano LR, Pelin A, Arrey TN, Damoc NE, Richards AL, Zhou Y, Lancaster NM, Peters-Clarke TM, Pashkova A, Jang GM, Eckhardt M, Quarmby ST, Zeller M, Hermanson D, Stewart H, Hock C, Makarov A, Zabrouskov V, Krogan NJ, Coon JJ, Swaney DL

Classical proteomics experiments offer high-throughput protein quantification but lack direct evidence of the spatial organization of the proteome, including protein-protein interaction (PPIs) networks. While affinity purification mass spectrometry (AP-MS) is the method of choice for generating these networks, technological impediments have stymied the throughput of AP-MS sample collection and therefore constrained the rate and scale of experiments that can be ... [more]

Throughput

• High Throughput

Curated By

• BioGRID