BAIT

ERBB4

ALS19, HER4, p180erbB4
erb-b2 receptor tyrosine kinase 4
GO Process (34)
GO Function (6)
GO Component (9)

Gene Ontology Biological Process

Homo sapiens
PREY

DLG1

DLGH1, SAP-97, SAP97, dJ1061C18.1.1, hdlg
discs, large homolog 1 (Drosophila)
GO Process (17)
GO Function (11)
GO Component (17)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The human PDZome: a gateway to PSD95-Disc large-zonula occludens (PDZ)-mediated functions.

Belotti E, Polanowska J, Daulat AM, Audebert S, Thome V, Lissitzky JC, Lembo F, Blibek K, Omi S, Lenfant N, Gangar A, Montcouquiol M, Santoni MJ, Sebbagh M, Aurrand-Lions M, Angers S, Kodjabachian L, Reboul J, Borg JP

Protein-protein interactions organize the localization, clustering, signal transduction, and degradation of cellular proteins and are therefore implicated in numerous biological functions. These interactions are mediated by specialized domains able to bind to modified or unmodified peptides present in binding partners. Among the most broadly distributed protein interaction domains, PSD95-disc large-zonula occludens (PDZ) domains are usually able to bind carboxy-terminal sequences ... [more]

Mol Cell Proteomics Sep. 01, 2013; 12(9);2587-603 [Pubmed: 23722234]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ERBB4 DLG1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID