FOXP1
Gene Ontology Biological Process
- T follicular helper cell differentiation [ISS]
- chemokine (C-C motif) ligand 2 secretion [IDA]
- endothelial cell activation [IMP]
- interleukin-21 secretion [ISS]
- macrophage activation [IDA]
- monocyte activation [IDA]
- negative regulation of B cell apoptotic process [IDA]
- negative regulation of androgen receptor signaling pathway [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- osteoclast development [IDA]
- osteoclast differentiation [IDA]
- positive regulation of endothelial cell migration [IMP]
- positive regulation of smooth muscle cell proliferation [IMP]
- regulation of defense response to bacterium [IDA]
- regulation of endothelial tube morphogenesis [IMP]
- regulation of inflammatory response [IDA]
- regulation of interleukin-1 beta secretion [IDA]
- regulation of interleukin-12 secretion [IDA]
- regulation of macrophage colony-stimulating factor production [IDA]
- regulation of monocyte differentiation [IDA]
- regulation of transcription from RNA polymerase II promoter [IBA]
- regulation of tumor necrosis factor production [IDA]
- response to lipopolysaccharide [IDA]
- transcription from RNA polymerase II promoter [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- nucleoplasm [IDA]
- nucleus [IDA]
CTBP1
Gene Ontology Biological Process
- chromatin organization involved in regulation of transcription [IMP]
- negative regulation of cell proliferation [TAS]
- negative regulation of histone H4 acetylation [IMP]
- negative regulation of histone acetylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription, DNA-templated [ISS]
- positive regulation of histone deacetylation [IMP]
- protein phosphorylation [TAS]
- regulation of cell cycle [IMP]
- viral genome replication [TAS]
- white fat cell differentiation [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A foundational atlas of autism protein interactions reveals molecular convergence
Translating high-confidence (hc) autism spectrum disorder (ASD) genes into viable treatment targets remains elusive. We constructed a foundational protein-protein interaction (PPI) network in HEK293T cells involving 100 hcASD risk genes, revealing over 1,800 PPIs (87% novel). Interactors, expressed in the human brain and enriched for ASD but not schizophrenia genetic risk, converged on protein complexes involved in neurogenesis, tubulin biology, ... [more]
Quantitative Score
- 0.980475735 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- APMS data generated in HEK293T by overexpression of Strep-tagged patient derived ASD variant. Intensity of the interaction between the WT protein and the hit protein was compared to the intensity of the interaction between the patient derived variant and hit protein. Significant interaction intensity changes were defined as those with a log2(fold-change) >= 1 and p <= 0.05. Strengthened interactions were denoted as 'Up', weakened interactions were denoted as 'Down' and unchanged interactions were denoted as 'Not significant' based on these scores. ASD variant: Bait protein name: L327P - Interaction Strength: not significant, Log2fc: 1.7128718216535, p-value: 0.0681970332044599
- APMS data generated in HEK293T by overexpression of Strep-tagged patient derived ASD variant. Intensity of the interaction between the WT protein and the hit protein was compared to the intensity of the interaction between the patient derived variant and hit protein. Significant interaction intensity changes were defined as those with a log2(fold-change) >= 1 and p <= 0.05. Strengthened interactions were denoted as 'Up', weakened interactions were denoted as 'Down' and unchanged interactions were denoted as 'Not significant' based on these scores. ASD variant: Bait protein name: R513C - Interaction Strength: not significant, Log2fc: -1.0367294668586, p-value: 0.23800749907673
- APMS data generated in HEK293T by overexpression of Strep-tagged patient derived ASD variant. Intensity of the interaction between the WT protein and the hit protein was compared to the intensity of the interaction between the patient derived variant and hit protein. Significant interaction intensity changes were defined as those with a log2(fold-change) >= 1 and p <= 0.05. Strengthened interactions were denoted as 'Up', weakened interactions were denoted as 'Down' and unchanged interactions were denoted as 'Not significant' based on these scores. ASD variant: Bait protein name: R513H - Interaction Strength: not significant, Log2fc: -1.04953694855269, p-value: 0.232758702392886
- Affinity purification-MS was used to capture high confidence wild-type protein interactors as defined by the thresholds: BFDR <= 0.05 and compPASS scores (rank_WD) >= 0.971. Baits are Strep-tagged proteins exogenously expressed in HEK 293T cells. ComPASS score: 0.980475735
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CTBP1 FOXP1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3300478 | |
| CTBP1 FOXP1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID