RFX3
Gene Ontology Biological Process
- cell maturation [ISS]
- cilium assembly [IBA, ISS]
- cilium-dependent cell motility [ISS]
- endocrine pancreas development [ISS]
- epithelial cilium movement involved in determination of left/right asymmetry [ISS]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of transcription, DNA-templated [IDA]
- positive regulation of type B pancreatic cell development [ISS]
- regulation of insulin secretion [ISS]
- regulation of transcription from RNA polymerase II promoter [IBA]
- regulation of transcription, DNA-templated [ISS]
- transcription from RNA polymerase II promoter [TAS]
- type B pancreatic cell differentiation [IBA]
Gene Ontology Molecular Function- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IBA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [TAS]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [NAS]
- transcription regulatory region DNA binding [IDA]
- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IBA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [TAS]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [NAS]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
VPS45
Gene Ontology Biological Process
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A foundational atlas of autism protein interactions reveals molecular convergence
Translating high-confidence (hc) autism spectrum disorder (ASD) genes into viable treatment targets remains elusive. We constructed a foundational protein-protein interaction (PPI) network in HEK293T cells involving 100 hcASD risk genes, revealing over 1,800 PPIs (87% novel). Interactors, expressed in the human brain and enriched for ASD but not schizophrenia genetic risk, converged on protein complexes involved in neurogenesis, tubulin biology, ... [more]
Quantitative Score
- 0.996312776 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- APMS data generated in HEK293T by overexpression of Strep-tagged patient derived ASD variant. Intensity of the interaction between the WT protein and the hit protein was compared to the intensity of the interaction between the patient derived variant and hit protein. Significant interaction intensity changes were defined as those with a log2(fold-change) >= 1 and p <= 0.05. Strengthened interactions were denoted as 'Up', weakened interactions were denoted as 'Down' and unchanged interactions were denoted as 'Not significant' based on these scores. ASD variant: Bait protein name: A508E - Interaction Strength: not significant, Log2fc: -0.0078693259825862, p-value: 0.991001392636603
- Affinity purification-MS was used to capture high confidence wild-type protein interactors as defined by the thresholds: BFDR <= 0.05 and compPASS scores (rank_WD) >= 0.971. Baits are Strep-tagged proteins exogenously expressed in HEK 293T cells. ComPASS score: 0.996312776
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RFX3 VPS45 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID