Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SARS-CoV-2 nsp13 suppresses hepatitis B virus replication by targeting cccDNA transcription.

Li A, Zhao K, Duan Y, Zhang B, Zheng Y, Zhu C, Chen Q, Liu W-B, Hui L, Xia Y, Cheng X

SARS-CoV-2 nonstructural protein 13 (nsp13) has been shown to selectively suppress the transcription of episomal DNA while sparing chromosomal DNA. Hepatitis B Virus (HBV) harbors covalently closed circular DNA (cccDNA), a form of viral episomal DNA found within infected hepatocyte nuclei. The persistence of cccDNA is the major cause of chronic HBV infection. In this study, we investigated the impact ... [more]

J Virol Nov. 19, 2024; 98(11);e0104224 [Pubmed: 39373477]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NSP13 CSNK1D
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
2799080

Curated By

  • BioGRID