BAIT

UBE2QL1

ubiquitin-conjugating enzyme E2Q family-like 1
UPS Project
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens
PREY

NPLOC4

NPL4
nuclear protein localization 4 homolog (S. cerevisiae)
UPS Project
GO Process (3)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage.

Koerver L, Papadopoulos C, Liu B, Kravic B, Rota G, Brecht L, Veenendaal T, Polajnar M, Bluemke A, Ehrmann M, Klumperman J, Jaeaettelae M, Behrends C, Meyer H

The autophagic clearance of damaged lysosomes by lysophagy involves extensive modification of the organelle with ubiquitin, but the underlying ubiquitination machinery is still poorly characterized. Here, we use an siRNA screening approach and identify human UBE2QL1 as a major regulator of lysosomal ubiquitination, lysophagy, and cell survival after lysosomal damage. UBE2QL1 translocates to permeabilized lysosomes where it associates with damage ... [more]

EMBO Rep Oct. 04, 2019; 20(10);e48014 [Pubmed: 31432621]

Throughput

  • High Throughput

Additional Notes

  • Proximity Label MS was carried out to identify high confidence protein interactors in damaged lysosomes with a fold-change threshold of log2 H/L greater than or equal to 1.5 and P-value < 0.05

Curated By

  • BioGRID