Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Quantitative proteomic data of flotillin-2 interactome within detergent resistant membranes of HeLa cells.

Hryniewicz-Jankowska A, Tracz M, Opielka I, Augoff K, Czogalla A, Sikorski AF

Flotillin-binding protein networks serve as scaffolds, organizing lipid rafts and facilitating the recruitment of other raft-associated proteins such as receptors and downstream signaling molecules to regulate various intracellular pathways, including those involved in cell proliferation, migration, and endocytosis. Flotillins belong to the SPFH (stomatin/prohibitin/flotillin/HflK/C) domain-containing protein family, also known as the prohibitin homology (PHB) domain, which enables membrane association via ... [more]

Data Brief Oct. 01, 2025; 62();111970 [Pubmed: 40896124]

Throughput

  • Low Throughput

Additional Notes

  • A p value less than or equal to 0.05 and fold-change (FCH) greater or equal to 2 thresholds were assumed as meaningful enrichment (logarithmic values: -log10(p)=1.25, log2(FCH)=1)
  • BioID

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HNRNPH1 FLOT2
Affinity Capture-RNA
Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

High-BioGRID
2872375

Curated By

  • BioGRID