Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Covariation MS uncovers a protein that controls cysteine catabolism.

Xiao H, Ordonez M, Fink EC, Covington TA, Woldemichael HB, Chen J, Jain MS, Rohatgi MH, Wei SM, Burger N, Sharif MA, Jan J, Wang Y, Petrocelli JJ, Blackmore K, Smythers AL, Zhang B, Gilbert M, Cheong H, Khetarpal SA, Smith A, Bogoslavski D, Lei Y, Vaites LP, McAllister FE, Van Bruggen N, Donovan KA, Huttlin EL, Mills EL, Fischer ES, Chouchani ET

The regulation of metabolic processes by proteins is fundamental to biology and yet is incompletely understood. Here we develop a mass spectrometry (MS)-based approach that leverages genetic diversity to nominate functional relationships between 285 metabolites and 11,868 proteins in living tissues. This method recapitulates protein-metabolite functional relationships mediated by direct physical interactions and local metabolic pathway regulation while nominating 3,542 previously ... [more]

Nature Sep. 17, 2025; (); [Pubmed: 40963025]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CUL5 LRRC58
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
CUL5 LRRC58
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
2222796
CUL5 LRRC58
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
3033679

Curated By

  • BioGRID