BAIT

BIRC5

API4, EPR-1
baculoviral IAP repeat containing 5
GO Process (19)
GO Function (14)
GO Component (12)
Homo sapiens
PREY

PPP1R13L

IASPP, NKIP1, RAI, RAI4
protein phosphatase 1, regulatory subunit 13 like
GO Process (0)
GO Function (3)
GO Component (3)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Localization matters: nuclear-trapped Survivin sensitizes glioblastoma cells to temozolomide by elevating cellular senescence and impairing homologous recombination.

Reich TR, Schwarzenbach C, Vilar JB, Unger S, Muehlhaeusler F, Nikolova T, Poplawski A, Baymaz HI, Beli P, Christmann M, Tomicic MT

To clarify whether differential compartmentalization of Survivin impacts temozolomide (TMZ)-triggered end points, we established a well-defined glioblastoma cell model in vitro (LN229 and A172) and in vivo, distinguishing between its nuclear and cytoplasmic localization. Expression of nuclear export sequence (NES)-mutated Survivin (SurvNESmut-GFP) led to impaired colony formation upon TMZ. This was not due to enhanced cell death but rather due ... [more]

Cell Mol Life Sci Jul. 01, 2021; 78(14);5587-5604 [Pubmed: 34100981]

Throughput

  • High Throughput

Curated By

  • BioGRID