BAIT

MRC1

SPAC694.06c
mediator of replication checkpoint 1
Schizosaccharomyces pombe (972h)
PREY

MCL1

slr3, SPAPB1E7.02c
DNA polymerase alpha accessory factor Mcl1
GO Process (3)
GO Function (2)
GO Component (3)
Schizosaccharomyces pombe (972h)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.

Yu J, Zhang Y, Fang Y, Paulo JA, Yaghoubi D, Hua X, Shipkovenska G, Toda T, Zhang Z, Gygi SP, Jia S, Li Q, Moazed D

Faithful transfer of parental histones to newly replicated daughter DNA strands is critical for inheritance of epigenetic states. Although replication proteins that facilitate parental histone transfer have been identified, how intact histone H3-H4 tetramers travel from the front to the back of the replication fork remains unknown. Here, we use AlphaFold-Multimer structural predictions combined with biochemical and genetic approaches to ... [more]

Cell Sep. 05, 2024; 187(18);5010-5028.e24 [Pubmed: 39094570]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MRC1 MCL1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-8.5669BioGRID
523383

Curated By

  • BioGRID