PREY

PPP3CA

2900074D19Rik, CN, Caln, Calna, CnA
protein phosphatase 3, catalytic subunit, alpha isoform
GO Process (22)
GO Function (10)
GO Component (9)
Mus musculus

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Proteomic Characterization of the Alzheimer's Disease Risk Factor BIN1 Interactome.

McMillan JD, Wang S, Wohlfahrt J, Guergues J, Stevens SM, Thinakaran G

The gene BIN1 is the second-largest genetic risk factor for late-onset Alzheimer's disease (LOAD). It is expressed in neurons and glia in the brain as cell-type-specific and ubiquitous isoforms. BIN1 is an adaptor protein that regulates membrane dynamics in many cell types. Previously, we reported that BIN1 predominantly localizes to presynaptic terminals in neurons and regulates presynaptic vesicular release. However, ... [more]

Mol Cell Proteomics Sep. 01, 2025; 24(9);101055 [Pubmed: 40835006]

Throughput

  • High Throughput

Additional Notes

  • Enriched proteins from BIN1 TurboID samples (Welch's t-test P<0.05, positive t-test difference with Z-score>1) from N2a cell culture.
  • Enriched proteins from BIN1 TurboID samples (Welch's t-test P<0.05, positive t-test difference with Z-score>1) from mouse brain tissue samples.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BIN1 PPP3CA
Co-localization
Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Low-BioGRID
3839475

Curated By

  • BioGRID