BAIT

FIP37

ATFIP37, FKBP12 interacting protein 37, AT3G54170
FKBP12 interacting protein 37
GO Process (0)
GO Function (1)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

RPL27AB

60S RIBOSOMAL PROTEIN L27A, F26F24.13, F26F24_13, RIBOSOMAL PROTEIN L27A, RPL27A, AT1G23290
60S ribosomal protein L27a-2
GO Process (4)
GO Function (1)
GO Component (7)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

TurboID-mediated proximity labeling for screening interacting proteins of FIP37 in Arabidopsis.

Li X, Wei Y, Fei Q, Fu G, Gan Y, Shi C

Proximity labeling was recently developed to detect protein-protein interactions and members of subcellular multiprotein structures in living cells. Proximity labeling is conducted by fusing an engineered enzyme with catalytic activity, such as biotin ligase, to a protein of interest (bait protein) to biotinylate adjacent proteins. The biotinylated protein can be purified by streptavidin beads, and identified by mass spectrometry (MS). ... [more]

Plant Direct Dec. 01, 2023; 7(12);e555 [Pubmed: 38111714]

Throughput

  • High Throughput

Curated By

  • BioGRID