BAIT

SMD2

Sm D2, L000004145, YLR275W
Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
GO Process (1)
GO Function (0)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

SMF1

SBS1, divalent metal ion transporter SMF1, L000001931, YOL122C
Divalent metal ion transporter; broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins
Saccharomyces cerevisiae (S288c)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Interconversion and mechanisms between Lsm-type and Sm-type heteroheptameric rings: implications for spliceosome evolution and RNA metabolism.

Mu L, Hou Y, Hu Y, Wang Y, Shen C, Luo Y, Su D, Zhang R

Eukaryotes harbor both Sm-type and Lsm-type heteroheptameric rings, which are essential in RNA metabolism. Despite their similar subunits and evolutionary ties, they interact with RNA in distinct ways, functioning as scaffolds and chaperones, respectively. However, the mechanistic basis of their evolutionary divergence remains unclear. Using the Sm ring (D1-D2-F-E-G-D3-B) and the Lsm2-8 ring, both of which form the cores of ... [more]

Nucleic Acids Res May. 22, 2025; 53(10); [Pubmed: 40433979]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SMD2 SMF1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1269BioGRID
2002048

Curated By

  • BioGRID