BAIT

EEF1A2

EEF1AL, EF-1-alpha-2, EF1A, HS1, STN, STNL
eukaryotic translation elongation factor 1 alpha 2
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens
PREY

EEF1B2

EEF1B, EEF1B1, EF1B
eukaryotic translation elongation factor 1 beta 2
GO Process (4)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

Publication

Trioxane-based MS-cleavable cross-linking mass spectrometry for profiling multimeric interactions of cellular networks.

Yu C, Novitsky E, Balasubramani SG, Wang X, Shen X, Yang Q, Rychnovsky S, Echeverria I, Huang L

Cross-linking mass spectrometry (XL-MS) is a powerful technology for mapping protein-protein interactions (PPIs) at the systems level. While bivalent cross-links are effective for defining protein interactions and structures, multivalent cross-links offer enhanced spatial resolution to facilitate characterization of heterogeneous protein complexes. However, their identification remains challenging due to fragmentation complexity and the vast expansion of database search space. Here, we present ... [more]

Nat Commun Jul. 01, 2025; 16(1);5585 [Pubmed: 40593561]

Throughput

  • High Throughput

Ontology Terms

  • hek-293 cell (BTO:0000007) [kidney cell line (BTO:0000067)]

Additional Notes

  • In vivo TSTO cross-linking of HEK293 cells
  • Supplementary Data 3

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EEF1A2 EEF1B2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.441BioGRID
241798
EEF1A2 EEF1B2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9962BioGRID
2236865
EEF1A2 EEF1B2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9876BioGRID
3051947
EEF1A2 EEF1B2
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.2911BioGRID
1261367
EEF1A2 EEF1B2
Cross-Linking-MS (XL-MS)
Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

High-BioGRID
-

Curated By

  • BioGRID