BAIT

VMS1

YDR049W
Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

LOS1

L000000950, YKL205W
Nuclear pore protein; involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm
GO Process (2)
GO Function (2)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Publication

Delayed protein translocation protects mitochondria against toxic CAT-tailed proteins.

Bertram N, Izawa T, Thoma F, Schwenkert S, Duvezin-Caubet S, Park SH, Wagener N, Devin A, Osman C, Neupert W, Mokranjac D

Ribosome-associated protein quality control (RQC) protects cells against the toxic effects of faulty polypeptides produced by stalled ribosomes. However, mitochondria are vulnerable to C-terminal alanyl and threonyl (CAT)-tailed proteins that are generated in this process, and faulty nuclear-encoded mitochondrial proteins are handled by the recently discovered mitoRQC. Here, we performed a genome-wide screen in yeast to identify additional proteins involved ... [more]

Mol Cell Nov. 06, 2025; 85(21);4082-4092.e7 [Pubmed: 41118763]

Throughput

  • High Throughput

Ontology Terms

  • viability (APO:0000111)

Additional Notes

  • Genetic complex
  • vms1/ ltn1 double mutant cells were mated with the yeast deletion library and screened for triple mutants that are viable

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
VMS1 LOS1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1896BioGRID
2093624

Curated By

  • BioGRID