An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Neddylation of PTEN regulates its nuclear import and promotes tumor development.

Xie P, Peng Z, Chen Y, Li H, Du M, Tan Y, Zhang X, Lu Z, Cui CP, Liu CH, He F, Zhang L

PTEN tumor suppressor opposes the PI3K/Akt signaling pathway in the cytoplasm and maintains chromosomal integrity in the nucleus. Nucleus-cytoplasm shuttling of PTEN is regulated by ubiquitylation, SUMOylation and phosphorylation, and nuclear PTEN has been proposed to exhibit tumor-suppressive functions. Here we show that PTEN is conjugated by Nedd8 under high glucose conditions, which induces PTEN nuclear import without effects on ... [more]

Cell Res Mar. 01, 2021; 31(3);291-311 [Pubmed: 33299139]

Throughput

Low Throughput

Additional Notes

#LPPI
Likely protein-protein interaction

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NEDD8 NAE1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Bennett EJ (2010)	High	-	BioGRID	691124
NEDD8 NAE1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Li T (2006)	High	-	BioGRID	438666
NEDD8 NAE1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Norman JA (2006)	Low	-	BioGRID	675481
NAE1 NEDD8	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Huang DT (2007)	Low	-	BioGRID	682422
NAE1 NEDD8	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Huang DT (2008)	Low	-	BioGRID	682420
NEDD8 NAE1	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Xiong C (2021)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

NEDD8

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

NAE1

Gene Ontology Biological Process

Gene Ontology Molecular Function

NEDD8 activating enzyme activity [IDA]protein binding [IPI]protein heterodimerization activity [IPI]ubiquitin protein ligase binding [IPI]