An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Genome-wide CRISPR screen identifies splicing factor SF3B4 in driving hepatocellular carcinoma.

Guo Y, Xu M, Xue H, Ding X, Wong AM, Lin N, Pu D, Wong AM, Wang X, Zhao H, Wong N

Although genome sequencings have recognized many cancer-associated genes in hepatocellular carcinoma (HCC), distinguishing their functional effect remains challenging. Leveraging on a genome-wide CRISPR knockout (KO) screening, we uncovered spliceosome factors as major survival essential genes in HCC and up-regulations of ferroptosis suppressors [particularly glutamate-cysteine ligase catalytic subunit (GCLC)] in lenvatinib resistance. Our KO screen in patient-derived HCC organoid showed splicing ... [more]

Sci Adv Oct. 10, 2025; 11(41);eadw7181 [Pubmed: 41071884]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
STAU1 SF3B4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Milev MP (2012)	High	-	BioGRID	-
STAU1 SF3B4	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Gonzalez Quesada Y (2022)	High	-	BioGRID	3528585

Curated By

BioGRID

Interaction Summary

SF3B4

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

STAU1