BAIT

SF3B4

AFD1, Hsh49, SAP49, SF3b49, RP11-196G18.20-002
splicing factor 3b, subunit 4, 49kDa
GO Process (5)
GO Function (2)
GO Component (3)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Genome-wide CRISPR screen identifies splicing factor SF3B4 in driving hepatocellular carcinoma.

Guo Y, Xu M, Xue H, Ding X, Wong AM, Lin N, Pu D, Wong AM, Wang X, Zhao H, Wong N

Although genome sequencings have recognized many cancer-associated genes in hepatocellular carcinoma (HCC), distinguishing their functional effect remains challenging. Leveraging on a genome-wide CRISPR knockout (KO) screening, we uncovered spliceosome factors as major survival essential genes in HCC and up-regulations of ferroptosis suppressors [particularly glutamate-cysteine ligase catalytic subunit (GCLC)] in lenvatinib resistance. Our KO screen in patient-derived HCC organoid showed splicing ... [more]

Sci Adv Oct. 10, 2025; 11(41);eadw7181 [Pubmed: 41071884]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SFPQ SF3B4
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
3204171

Curated By

  • BioGRID