An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Dominant spinal muscular atrophy linked mutations in the cargo binding domain of BICD2 result in altered interactomes and dynein hyperactivity.

Neiswender H, Pride JE, Veeranan-Karmegam R, Allen P, Neiswender G, Prabakar A, Hao C, Fan X, Gonsalvez GB

Cytoplasmic dynein-1 (dynein) is responsible for the transport of most cellular cargo towards the minus end of microtubules. Dynein activation requires the multi-subunit dynactin complex and an activating cargo adaptor. The adaptors serve to link dynein with cargo and to fully activate the motor. Mutations in one of these activating adaptors, Bicaudal-D2 (BICD2), are associated with a neurodegenerative disease called ... [more]

Elife Dec. 03, 2025; 14(); [Pubmed: 41334889]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
BICD2 VPS33A	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.7747	BioGRID	3195321

Curated By

BioGRID

Interaction Summary

BICD2

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rab GTPase binding [ISS]protein binding [IPI]

Gene Ontology Cellular Component

VPS33A