BAIT

STAT3

ADMIO, APRF, HIES
signal transducer and activator of transcription 3 (acute-phase response factor)
GO Process (27)
GO Function (10)
GO Component (5)

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Analysis of the STAT3 interactome using in-situ biotinylation and SILAC.

Blumert C, Kalkhof S, Brocke-Heidrich K, Kohajda T, von Bergen M, Horn F

Signal transducer and activator of transcription 3 (STAT3) is activated by a variety of cytokines and growth factors. To generate a comprehensive data set of proteins interacting specifically with STAT3, we applied stable isotope labeling with amino acids in cell culture (SILAC). For high-affinity pull-down using streptavidin, we fused STAT3 with a short peptide tag allowing biotinylation in situ (bio-tag), ... [more]

J Proteomics Dec. 06, 2013; 94();370-86 [Pubmed: 24013128]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
STAT3 COPA
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3319156

Curated By

  • BioGRID