BAIT

APN1

DNA-(apurinic or apyrimidinic site) lyase APN1, L000000096, YKL114C
Major apurinic/apyrimidinic endonuclease; 3'-repair diesterase; involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine; genetically interacts with NTG1 to maintain mitochondrial genome integrity
Saccharomyces cerevisiae (S288c)
PREY

RAD9

chromatin-binding protein RAD9, L000001562, YDR217C
DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M, plays a role in postreplication repair (PRR) pathway; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; Rad9p Chk1 Activating Domain (CAD) is phosphorylated at multiple sites by Cdc28p/Clb2p
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Base excision repair and homologous recombination are required for prevention of a chronic DNA damage response in Saccharomyces cerevisiae.

Nepal Y, Ahmed S, Berry AM, Mahmood A, Holland CL, Lewis LK

The chromosomes within eukaryotic cells experience many types of damage that are generated naturally via endogenous processes. The specific pathways that are most critical for repair of such endogenously produced DNA lesions have not been identified. Previous work revealed that budding yeast mutants deficient in double-strand break repair exhibit a persistent DNA damage checkpoint response leading to chronically high levels ... [more]

DNA Repair (Amst) Dec. 16, 2025; 157();103915 [Pubmed: 41411878]

Throughput

  • Low Throughput

Ontology Terms

  • mitotic cell cycle (APO:0000072)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
APN1 RAD9
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
617497
APN1 RAD9
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
863641

Curated By

  • BioGRID