An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

Publication

Stress-specific NONO interactomes reveal a key role of Hsp70 chaperone activity in regulation of paraspeckle formation.

Odonkor I, Shrestha BK, Nielsen SR, Kournoutis A, Bjorlo IE, Sajib SD, Hedberg A, Gronset TA, Larsen KB, Brun JA, Knutsen E, Lellahi SM, Perander M

Paraspeckles are stress-induced nuclear RNA-protein condensates that assemble on the long non-coding RNA NEAT1. Their increased formation under certain cellular circumstances has gained growing interest due to their association with serious human diseases such as neurodegenerative disorders and cancer. The biological functions of paraspeckles still appear obscure, but increasing evidence suggests that they contribute to regulation of gene expression by ... [more]

J Cell Sci Dec. 19, 2025; (); [Pubmed: 41416371]

Throughput

High Throughput

Additional Notes

interaction enriched under SFN (sulforaphane) treatment in a Neat1KO background

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ELAVL1 NONO	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Abdelmohsen K (2009)	High	-	BioGRID	664107
ELAVL1 NONO	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Youn JY (2018)	High	-	BioGRID	2813562

Curated By

BioGRID

Interaction Summary

NONO

Gene Ontology Biological Process

Gene Ontology Molecular Function

core promoter binding [ISS]identical protein binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

ELAVL1

Gene Ontology Biological Process

Gene Ontology Molecular Function

AU-rich element binding [IDA]RNA binding [IDA]double-stranded RNA binding [IDA]mRNA 3'-UTR AU-rich region binding [IDA]mRNA 3'-UTR binding [ISS]mRNA binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase binding [IPI]