BAIT

AGO2

EIF2C2, Q10
argonaute RISC catalytic component 2
Homo sapiens
PREY

YTHDF3

YTH N(6)-methyladenosine RNA binding protein 3
GO Process (0)
GO Function (3)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Systematic identification of mRNAs recruited to argonaute 2 by specific microRNAs and corresponding changes in transcript abundance.

Hendrickson DG, Hogan DJ, Herschlag D, Ferrell JE, Brown PO

microRNAs (miRNAs) are small non-coding RNAs that regulate mRNA stability and translation through the action of the RNAi-induced silencing complex (RISC). Our current understanding of miRNA function is inferred largely from studies of the effects of miRNAs on steady-state mRNA levels and from seed match conservation and context in putative targets. Here we have taken a more direct approach to ... [more]

PLoS One May. 07, 2008; 3(5);e2126 [Pubmed: 18461144]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
YTHDF3 AGO2
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Low-BioGRID
2817374

Curated By

  • BioGRID