An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Systematic identification of mRNAs recruited to argonaute 2 by specific microRNAs and corresponding changes in transcript abundance.

Hendrickson DG, Hogan DJ, Herschlag D, Ferrell JE, Brown PO

microRNAs (miRNAs) are small non-coding RNAs that regulate mRNA stability and translation through the action of the RNAi-induced silencing complex (RISC). Our current understanding of miRNA function is inferred largely from studies of the effects of miRNAs on steady-state mRNA levels and from seed match conservation and context in putative targets. Here we have taken a more direct approach to ... [more]

PLoS One May. 07, 2008; 3(5);e2126 [Pubmed: 18461144]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AGO2 PDCD4	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Qiu R (2020)	High	-	BioGRID	2970140

Curated By

BioGRID

Interaction Summary

AGO2

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA 7-methylguanosine cap binding [IDA]endoribonuclease activity [EXP]endoribonuclease activity, cleaving siRNA-paired mRNA [IDA]poly(A) RNA binding [IDA]protein binding [IPI]siRNA binding [IDA]translation initiation factor activity [NAS]

Gene Ontology Cellular Component

PDCD4