An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Differentiation-dependent proximity proteomics identifies novel host factors linked to HPV16 E2 function.

James CD, Youssef A, Prabhakar AT, Roe JD, Lu E, Witt A, Giri S, Bristol ML, Bridy P, Wang X, Rijal A, Lyons C, Morgan IM

Human papillomavirus 16 (HPV16) is a causative agent of oropharyngeal, cervical, and anogenital cancers. The viral E2 protein is essential for viral genome replication, transcriptional regulation, episome maintenance, and activation of the host DNA damage response. Despite its central role, the full network of HPV16 E2 interactions with host proteins remains incompletely defined, particularly under differentiating conditions, which support the ... [more]

mBio Jan. 12, 2026; ();e0319425 [Pubmed: 41524403]

Throughput

High Throughput

Ontology Terms

keratinocyte cell line (BTO:0000375)
keratinocyte (CL:0000312)
skin (BTO:0001253)

Additional Notes

TurboID
interaction observed under undifferentiated monolayer conditions

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PPP1CC TOPBP1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Fardilha M (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

TOPBP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]protein C-terminus binding [TAS]protein binding [IPI]

Gene Ontology Cellular Component

PPP1CC