BAIT

TOPBP1

TOP2BP1
topoisomerase (DNA) II binding protein 1
Fanconi Anemia Project
Human Papilloma Virus (HPV) Project
Homo sapiens
PREY

DTD1

C20orf88, DUE-B, DUEB, HARS2, bA379J5.3, bA555E18.1, pqn-68
D-tyrosyl-tRNA deacylase 1
GO Process (2)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Differentiation-dependent proximity proteomics identifies novel host factors linked to HPV16 E2 function.

James CD, Youssef A, Prabhakar AT, Roe JD, Lu E, Witt A, Giri S, Bristol ML, Bridy P, Wang X, Rijal A, Lyons C, Morgan IM

Human papillomavirus 16 (HPV16) is a causative agent of oropharyngeal, cervical, and anogenital cancers. The viral E2 protein is essential for viral genome replication, transcriptional regulation, episome maintenance, and activation of the host DNA damage response. Despite its central role, the full network of HPV16 E2 interactions with host proteins remains incompletely defined, particularly under differentiating conditions, which support the ... [more]

mBio Jan. 12, 2026; ();e0319425 [Pubmed: 41524403]

Throughput

  • High Throughput

Ontology Terms

  • keratinocyte cell line (BTO:0000375)
  • keratinocyte (CL:0000312)
  • skin (BTO:0001253)

Additional Notes

  • TurboID
  • interaction observed under undifferentiated monolayer conditions

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DTD1 TOPBP1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
TOPBP1 DTD1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID