BAIT

RAC1

Rac-1, TC-25, p21-Rac1, MIG5
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
GO Process (41)
GO Function (6)
GO Component (10)

Gene Ontology Biological Process

Homo sapiens
PREY

CNP

CNP1
2',3'-cyclic nucleotide 3' phosphodiesterase
GO Process (2)
GO Function (1)
GO Component (7)
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Proximity-based proteomics (BioID) uncovers the Rho GTPase interactome in kidney podocytes.

Ibrahim S, Matsuda J, Nurcombe ZW, Boulais J, Aoudjit L, Foxman E, Kazan C, Suzuki S, Leclerc S, Shimada N, Kitzler T, Cote JF, Takano T

Podocyte injury causes proteinuria. Rho GTPases play critical roles in regulating the podocyte cytoskeleton, and their alteration leads to foot process effacement. Yet, their signaling networks remain poorly understood.To address this, we mapped the interactomes of RhoA, Rac1, and Cdc42 in human podocytes using proximity-dependent biotin identification (BioID) labeling.Our BioID analysis detected a total of 1927 interactions with AvgP ? ... [more]

Front Cell Dev Biol Nov. 27, 2025; 13();1625950 [Pubmed: 41306285]

Throughput

  • High Throughput

Additional Notes

  • Rac1G15A

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RAC1 CNP
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3336881

Curated By

  • BioGRID