BAIT

GET1

MDM39, YGL020C
Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance
GO Process (2)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

IST2

YBR086C
Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Ist2 is a phospholipid scramblase that links lipid transport at the ER to organelle homeostasis.

Gobbi Sebinelli H, Syska C, Razmazma H, Albanese V, Dias Araujo AR, Hilpert C, Montigny C, Jaxel C, Tchamba M, Belingar K, D'Ambrosio JM, Monticelli L, Lenoir G, Copic A

Lipid scramblases allow passive flip-flop of phospholipids between bilayer leaflets, thereby promoting membrane symmetry. At the endoplasmic reticulum (ER), where phospholipid synthesis is restricted to one leaflet, scramblase activity should be essential for equilibrated membrane growth. The yeast protein Ist2 contains an ER domain and a cytosolic tail that binds the plasma membrane and participates in the transfer of phosphatidylserine. ... [more]

J Cell Biol Feb. 02, 2026; 225(2); [Pubmed: 41474610]

Throughput

  • Low Throughput

Ontology Terms

  • vegetative growth (APO:0000106)
  • temperature sensitive growth (APO:0000092)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
IST2 GET1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.0827BioGRID
2428185

Curated By

  • BioGRID