An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

E2 variants for probing E3 ubiquitin ligase activities.

Du J, Andree GA, Horn-Ghetko D, Stier L, Singh J, Kostrhon S, Kiss L, Mann M, Sidhu SS, Schulman BA

E3 ligases partner with E2 enzymes to regulate vast eukaryotic biology. The hierarchical nature of these pairings, with >600 E3s and ~40 E2s in humans, necessitates that E2s cofunction with numerous different E3s. Here, focusing on E3s in the RING-between-RING (RBR) family and their partner UBE2L3 and UBE2D-family E2s, we report an approach to interrogate selected pathways. We screened phage-displayed ... [more]

Proc Natl Acad Sci U S A Jan. 06, 2026; 123(1);e2524899122 [Pubmed: 41481455]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MID1 UBE2L3	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Luck K (2020)	High	-	BioGRID	2707084
UBE2L3 MID1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Markson G (2009)	High	4	BioGRID	-

Curated By

BioGRID

Interaction Summary

UBE2L3

Gene Ontology Biological Process

Gene Ontology Molecular Function

enzyme binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]transcription coactivator activity [IDA]ubiquitin conjugating enzyme activity [IDA]ubiquitin protein ligase binding [IPI]ubiquitin-protein transferase activator activity [IGI]ubiquitin-protein transferase activity [IDA, IMP, TAS]

Gene Ontology Cellular Component

MID1