An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

ESCRT-dependent STING degradation inhibits steady-state and cGAMP-induced signalling.

Gentili M, Liu B, Papanastasiou M, Dele-Oni D, Schwartz MA, Carlson RJ, Al'Khafaji AM, Krug K, Brown A, Doench JG, Carr SA, Hacohen N

Stimulator of interferon genes (STING) is an intracellular sensor of cyclic di-nucleotides involved in the innate immune response against pathogen- or self-derived DNA. STING trafficking is tightly linked to its function, and its dysregulation can lead to disease. Here, we systematically characterize genes regulating STING trafficking and examine their impact on STING-mediated responses. Using proximity-ligation proteomics and genetic screens, we ... [more]

Nat Commun Feb. 04, 2023; 14(1);611 [Pubmed: 36739287]

Throughput

High Throughput

Additional Notes

Proximity label MS was carried out to identify high confidence protein interactors within 6h of cGAMP stimulation with an adj. p-value<0.07

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TMEM173 FKBP15	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.8888	BioGRID	3187295

Curated By

BioGRID

Interaction Summary

TMEM173

Gene Ontology Biological Process

Gene Ontology Molecular Function

cyclic-GMP-AMP binding [ISS]cyclic-di-GMP binding [IDA]identical protein binding [IPI]protein binding [IPI]protein homodimerization activity [IDA]protein kinase binding [IDA, IPI]transcription factor binding [IDA]

Gene Ontology Cellular Component

FKBP15