BAIT

GAPDH

G3PD, GAPD, HEL-S-162eP, CDABP0047
glyceraldehyde-3-phosphate dehydrogenase
Homo sapiens
PREY

TRIM23

ARD1, ARFD1, RNF46
tripartite motif containing 23
UPS Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Tobacco Smoking Rewires Cell Metabolism by Inducing GAPDH Succinylation to Promote Lung Cancer Progression.

Wang K, Li J, Zhang H, Ma H, Cui HY, Ju HQ, Zhang J, Ma QZ, Zhao M, Zeng QM, Zou J, Sun XX, Nan G, Qian M, Jing L, Li Y, Xiong CF, Yang QZ, Wang H, Jiang JL, Chen ZN, Chen L, Huang W

Patient behavior and physiology can directly affect cancer metabolism. Smoking is the leading risk factor for non-small cell lung cancer (NSCLC). In this study, we identified that smoking modulates lung cancer cell metabolism through altered protein post-translational modification. Proteomic analyses identified elevated K251 succinylation (K251-Su) of GAPDH, a key enzyme in glycolysis, in NSCLC samples, and GAPDH K251-Su was significantly ... [more]

Cancer Res Aug. 01, 2025; 85(15);2838-2857 [Pubmed: 40366632]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TRIM23 GAPDH
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3413373

Curated By

  • BioGRID