BAIT

COX5B

COXVB
cytochrome c oxidase subunit Vb
Homo sapiens
PREY

COA3

CCDC56, MITRAC12, HSPC009
cytochrome c oxidase assembly factor 3
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

Publication

Suborganelle-Specific Protein Complex Analysis Enabled by in Vivo Cross-Linking Coupled with Proximal Labeling.

An Y, Zhao Q, Gong Z, Zhao L, Li Y, Liang Z, Zou P, Zhang Y, Zhang L

The identification of the structure of protein complexes in the subcellular niche of cells is necessary to understand their diverse functions. In this study, we developed a suborganelle proteome labeling assisted in vivo cross-linking (SubPiXL) strategy to identify regional protein conformations and interactions in living cells. Due to the mitochondria's functional importance and well-defined compartmental partitions, the specific conformations and ... [more]

Anal Chem Sep. 06, 2022; 94(35);12051-12059 [Pubmed: 36004751]

Throughput

  • High Throughput

Additional Notes

  • Inter-protein links by suborganelle proteome labeling assisted in vivo cross-linking (SubPiXL)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
COA3 COX5B
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9457BioGRID
3126771
COX5B COA3
Cross-Linking-MS (XL-MS)
Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

High-BioGRID
-

Curated By

  • BioGRID