BAIT

PFK2

6-phosphofructokinase subunit beta, L000001405, YMR205C
Beta subunit of heterooctameric phosphofructokinase; involved in glycolysis; indispensable for anaerobic growth; activated by fructose-2,6-bisphosphate and AMP; mutation inhibits glucose induction of cell cycle-related genes
GO Process (2)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

MRPL6

mitochondrial 54S ribosomal protein YmL16, YmL16, L000001161, YHR147C
Mitochondrial ribosomal protein of the large subunit
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

The yeast phosphofructokinase ?-subunit has RNA unwinding activity and modulates cell cycle progression.

Albihlal WS, Matia-Gonzalez AM, Schmidt T, Mayer W, Bryant J, Bushell M, Niessing D, Barberis M, Schmidt A, Heinisch JJ, Gerber AP

Phosphofructokinase (PFK) is a rate-limiting glycolytic enzyme that also possesses an unexplored RNA binding activity. Here, we show that the ?- and ?-subunits of yeast PFK, encoded by PFK1 and PFK2, respectively, bind hundreds of functionally related messenger RNAs (mRNAs) in cells, including one's coding for proteins involved in the regulation of mitotic cell cycle. Both Pfk1p and Pkf2p directly bind ... [more]

Nucleic Acids Res Feb. 24, 2026; 54(5); [Pubmed: 41816908]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MRPL6 PFK2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1411BioGRID
2128787

Curated By

  • BioGRID